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  • Last update: 4 years ago
  • Total downloads: 3
  • Operating system: WinXP, Win Vista, Win All, Windows 7, Windows XP X64, Windows Vista64, Windows 7 x64, Windows 8
  • Publisher: Johannes & Mat
See full specifications

Flexbar for Windows 2.32 iconFlexbar for Windows Publisher's description

Flexible barcode and adapter removal for sequencing platforms

Flexbar preprocesses high-throughput sequencing data efficiently. It demultiplexes barcoded runs and removes adapter sequences. Moreover, trimming and filtering features are provided. Flexbar increases mapping rates and improves genome and transcriptome assemblies. It supports next-generation sequencing data in fasta/q and csfasta/q format from Illumina, Roche 454, and the SOLiD platform.

Parameter names changed in Flexbar. Please review scripts. The recent months, default settings were optimised, several bugs were fixed and various improvements were made, e.g. revamped command-line interface, new trimming modes as well as lower time and memory consumption.


Demultiplexes barcoded sequencing runs
Detects and removes adapter sequences
Uses exact global alignment with free end-gaps
Supports paired reads and separate barcode reads
Color and letter space sequencing data
Filtering with respect to uncalled bases
Trimming based on quality scores
Extensive logging features, e.g. alignments
Detailed documentation on manual page
Galaxy tool definition available in Tool Shed
Sequence analysis based on SeqAn library
Parallel computation via TBB library.

System Requirements:

No special requirements.
Program Release Status: New Release
Program Install Support: Install and Uninstall

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